In hygienic-technical regulations, among other things, requirements are also placed on materials which are installed in HVAC systems or evaporative cooling systems. For example, it is required that materials in the air-conducting area are corrosion-resistant or, if they are plastics, that they cannot be metabolized by microbes. Whether plastic materials meet this requirement can be tested, for example, according to DIN EN ISO 846 procedure A and C. If fungicides are used in the materials, the effectiveness can be tested using Procedure B.
The chemical resistance of materials to various cleaning agents and disinfectants is also relevant, or cleaning tests to show that plant surfaces can be cleaned and disinfected. Furthermore, the emission behavior of materials is also of interest.
DIN EN ISO 846 "Determination of the effect of microorganisms on plastics" Procedure A, B and C
Method A is used to assess the basic resistance of plastics to fungal attack in the absence of organic contaminants.
Method B is suitable for testing the fungistatic or fungicidal effectiveness of a material.
Method C is used to assess the principal resistance of plastics to bacterial attack in the absence of organic impurities.
For the test according to DIN EN ISO 846 "Determination of the effect of microorganisms on plastics", the test specimens are inoculated under defined conditions with test fungi for method A and with a bacterium for method C and incubated in a nutrient-free environment. After 4 weeks of incubation, the microbial growth is assessed visually.
For Procedure A, the following test fungi are used:
- Aspergillus niger
- Penicillium pinophillum
- Paecilomyces variotii
- Trichoderma virens
- Chaetomium globosum
For procedure C, the following bacterial strain is used:
For the tests we need 30 test specimens per material. The test specimens must have a size of 50 mm x 50 mm x ≤ 6 mm. For paint or varnish materials, you should ideally use glass or stainless steel platelets as a coating base.